二甲基亚砜(DMSO)Dimethyl Sulfoxide (DMSO)

• 产品编号：0219605591      品牌：MP       原厂货号：0219605591
• 产品分类：细胞,细胞培养与转染试剂 > 其它细胞培养试剂 > 细胞冻存液
• 应用分类：暂定分类 > 细胞冻存制剂
  　　　　　暂定分类 > 细胞冻存制剂 > 细胞培养冻存培养基

描述：

Dimethyl Sulfoxide
Cell Culture Reagent
Purity: ≥99%
Ideal for use as a cryoprotectant.
NOT FOR HUMAN USE!

Structure:



Molecular Formula: C₂H₆SO (for non-heavy labels)

Molecular Weight: 78.13 (non-heavy labels)

CAS #: 67-68-5

Synonyms: DMSO; Methyl sulfoxide; Methyl sulphoxide; Sulfinylbismethane

Physical Description: Clear colorless liquid to solid. The melting point is approximately 18°C so the product may appear anywhere from the liquid phase to the solid phase when in the pure form. By heating the solid form to approximately 30°C, the product can be melted without harming it's stability. DMSO supercools easily; melts at room temperature slowly.

Solubility: Soluble in water, methanol, acetone, ether, benzene, chloroform.¹ To prepare sterile solutions use a a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Stability: A thermally stable compound. DMSO is stable up to 100°C in alkaline, acidic and neutral conditions. It is stable in neutral or alkaline conditions at temperatures approaching its boiling point of 189°C. DMSO can be heated to 150°C for 24 hours with less than 0.1% loss in purity.⁵

DMSO is very hygroscopic.¹

Density: Approximately 1.10 g/ml

Autoprotolysis constant: Approximately 33 at 25°C.⁷

Dielectric constant: 45 ¹

Description: A dipolar, aprotic solvent.¹ Has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.^3,8 

Typical Uses:

• • Used to enhance dermal absorption of many chemicals.
  • A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers.
  • Used in antifreeze or hydraulic fluids.
  • As a cryopreservative for cell cultures.⁵
  • Used in the oxidation of thiols and disulfides to sulfonic acids.⁴
  • Used as a PCR cosolvent to help improve yields, especially in long PCR.

Plastic Compatibility:⁶

产品名称：二甲基亚砜，又称二甲亚砜

结构简式：（CH3)2-S-O
英文名：Dimethyl sulfoxide
别名：Dimethylsulfoxide; Methyl sulfoxide; Sulfinylbis (methane); DMSO；Me2SO　
用途　　二甲基亚砜是一种既溶于水又溶于有机溶剂的极为重要的非质子极性溶剂。广泛用作溶剂和反应试剂，具有很高的选择抽提能力。在医药工业中二甲基亚砜还有直接用作某些药物的原料及载体。二甲基亚砜本身有消炎止痛，利尿，镇静等作用。
在医药生产中的应用
　　DMSO 作为反应溶剂在医药中间体合成中应用很多。如：用氟化钾与3，4―二氯硝基苯在DMSO中反应制得氟氯苯胺，被广泛用于氟哌酸生产以及三氟硝基甲苯、氧氟沙星等含氟药物。

在防冻剂中的应用
　　纯DMSO 的冰点是18。45℃，含水40%的DMSO在-60℃不冻，而且DMSO与水、雪混合时放热。这种性质使DMSO可作为汽车防冻液、刹车油、液压液组分。乙二醇防冻液在超过-40℃低温时已不适用。而且比DMSO沸点低，有毒，易生产气阻。DMSO防冻液在北部严寒地区用于除冰剂，涂料、各种乳胶的防冻剂，汽油、航煤的防冰剂，骨髓、血液、 低温保存的防冻剂等

在细胞冻存中的应用
　　二甲基亚砜是一种重要的渗透型细胞保护剂。在深低温（零下200度）保存细胞时冻存过程中，为防止细胞内液冰晶形成、渗透压改变、细胞结构紊乱等导致的损伤，有必要使用含有DMSO冷冻保护剂。DMSO能够快速穿透细胞膜进入细胞中，降低冰点、延缓冻存过程，同时提高细胞内离子浓度，减少细胞内冰晶的形成，从而减少细胞损伤。深低温时二甲基亚砜的细胞毒性受到抑制。复苏时动作要快，尽快洗掉二甲基亚砜，否则会造成对细胞严重的毒性。二甲基亚砜（DMSO）是目前最好的细胞冻存保护剂，但也是一种以细胞毒性很大的化学试验剂。研究结果表明，培养液中DMSO浓度为10%时，细胞生长抑制率近100%；1‰浓度时抑制率为35%，即使是0.04‰的浓度，DMSO对细胞的生长也有不利的影响。

原厂资料：

注意事项：

Caution: Rapidly absorbed through skin and mucous membranes.

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