Phosphate is involved in many biological reactions. For example, phosphatases, ATPases and several other enzymes catalyze reactions in which inorganic phosphate (Pi) is released from a substrate. This PhosphoWorks™ Phosphate Assay Kit has been developed for measuring the activity of any Pi-generating enzyme. The kit is formulated to give sensitive detection of Pi, providing an alternative to hazardous radioactive methods and other less sensitive colorimetric assays. The measurement of Pi is based on the change in absorbance of a malachite green derivative in the presence of molybdate. Unlike other malachite dye formulations, this kit gives a completely stable end-point signal that is not prone to precipitation.
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