Description:
The Low Range ssRNA Ladder is a set of 6 RNA molecules produced by in vitro transcription of a mixture of 6 linear DNA templates. The ladder sizes are: 1000, 500, 300, 150, 80 and 50 bases. The 300 base fragment is at double intensity to serve as a reference band. This ladder is suitable for use as an ssRNA size standard on denaturing polyacrylamide-urea gels, and on denaturing or native agarose gels.
Usage Recommendation:
This marker was not designed for precise quantification of ssRNA mass.
Denaturing vs. Native Agarose Gels:
It is common practice to electrophorese RNA on a fully denaturing agarose gel, such as one containing formaldehyde (1). However, in many cases it is possible to run RNA on a native agarose gel and obtain suitable results. In fact, it has been demonstrated that treatment of RNA samples in a denaturing buffer maintains the RNA molecules in a denatured state, during electrophoresis, for at least 3 hours (2,3). The use of native agarose gels eliminates problems associated with toxic chemicals and the difficulties encountered when staining and blotting formaldehyde gels. Reagents Supplied:
ssRNA Ladder Loading Buffer (2X)
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