Description: This Mouse IL-17E ELISA Ready-SET-Go! reagent set contains all of the necessary buffers and reagents to perform enzyme-linked immunosorbent assays. The set has been optimized for the accurate and precise measurement of mouse IL-17E in serum and tissue culture supernatant samples. Serum samples must be diluted 8-fold in the provided sample diluent prior to evaluation in this assay.
IL-17E, or IL-25, was first identified as an IL-17 family member on the basis of sequence homology. It was originally believed to be a Th2 cytokine based on the detection of mRNA transcripts in polarized Th2 cells, but expression was later observed in epithelial cells, eosinophils, and mast cells. Later studies confirmed the role of IL-17E as a potent inducer of type 2 immune responses. IL-17E signaling induces the differentiation of CD4+ T cells towards the Th2 lineage and upregulates expression of Th2 cytokines such as IL-4, IL-5, and IL-13. Administration of IL-17E also promotes symptoms of allergic asthma, such as eosinophil infiltration, airway hyperreactivity, and high circulating levels of IgE. IL-17E binds to IL-17BR, a receptor shared with IL-17B, and activates NF kappa-B pathways.
Components
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples Coating Buffer. 10X PBS ELISA Coating Buffer Assay Diluent. 5X Concentrated Sample Diluent B. 12 mL of a 1X solution per plate Detection Enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards. 96-well Plates. Corning Costar flat-bottom plates (included with Cat. #s ending in -22 and -86)
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