Centri-Spin™ columns provide excellent recovery of RNA and DNA fragments for a variety of purification applications. Use Centri-Spin™ columns to remove primers, probes, and dNTPs from DNA, RNA, PCR and end-labeled products.
• Processes samples faster than ultrafiltration columns
• Rapid and efficient separations
• Permits user to determine buffer composition
• Convenient 20-50 µL sample size
• Dry gel format
• Room Temperature Storage
• Microcentrifuge format
Excellent Recovery
Centri-Spin™ columns provide excellent recovery of DNA fragments with sizes greater than 10, 20 or 40 base pairs (depending on kit), while removing >98% of salts, NTP's and other low-molecular-weight impurities. Proteins, peptides, and protein conjugates >5 kD can also be efficiently separated⁄desalted from unwanted low-molecular-weight impurities.
Designed for Performance
The column design is based on the description of gel filtration for the purification of DNA from nick translation reactions by Sambrook, et.al. Each Centri-Spin™ consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
Versatile
The column gel can be hydrated with either reagent-grade water or a suitable buffer, and spun in a microcentrifuge or swinging-bucket centrifuge to remove the intersititial fluid. After your sample is applied, the column is spun again to remove the low-molecular-weight components, leaving you not only with a purified sample, but one exchanged into the buffer of your choice.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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