TOPO TA/ DH5A (25) 25 RXN

产品编号：K452001 品牌：invitrogen 原厂货号：K452001
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > PCR克隆载体
应用分类：暂定分类 > TOPO® TA Cloning > TOPO® Cloning Kit by Application

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2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

其它组分：

pCR?.1-TOPO®or pCR®II-TOPO®【包装：20 μl，，运保温度：-20°C】

10X PCR Buffer【包装：100 μl，，运保温度：-20°C】

Salt Solution【包装：50 μl，，运保温度：-20°C】

dNTP Mix【包装：10 μl，，运保温度：-20°C】

M13 Forward (-20) Primer【包装：20 μl，，运保温度：-20°C】

M13 Reverse Primer【包装：20 μl，，运保温度：-20°C】

Control Template【包装：10 μl，，运保温度：-20°C】

Control PCR Primers【包装：10 μl，，运保温度：-20°C】

Water【包装：1 ml，，运保温度：-20°C】

S.O.C. Medium【包装：6 ml，，运保温度：-80°C】

TOP10, Mach1.-T1R, DH5α.-T1R, or TOP10F′ cells orTOP10 cells 【包装：21 x 50 μl，，运保温度：-80°C】

pUC19 Control DNA【包装：50 μl，，运保温度：-80°C】

PureLink. Quick Plasmid Miniprep Kit【包装：1 Kit，，运保温度：4°C】

描述：

TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR®-TOPO® Vectors include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR®2.1-TOPO®) or T7 and SP6 (pCR®II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants

TOPO® TA Cloning® Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.

注意事项：

Each TOPO® TA Cloning® Kit contains linearized and topoisomerase I-activated pCR®2.1-TOPO® or pCR®II-TOPO® Vector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot® Chemically Competent or Electrocomp™ E. coli, S.O.C. Medium, and a supercoiled control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.