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pNEB206A Linearized Vector

 
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描述:

Description: 
Linearized pNEB206A Vector is supplied with the USER™ Friendly Cloning Kit. pNEB206A has been linearized within the Multiple Cloning Site to produce 8-nucleotide, 3´ single-stranded extensions on both vector ends. The single-stranded extensions are not complementary; this prevents the vector termini from re-annealing to form transformable circular DNA and also allows control over the orientation of the inserted PCR product. pNEB206A linearized vector is 2,706 base pairs in length.

Source:
pNEB206A is isolated from E. coli by a standard purification procedure, digested to completion with XbaI and nicked with Nt.BbvCI. The DNA is phenol extracted and resuspended in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Concentration:
20 μg/ml


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