质粒大提，阴离子交换树脂法，用于转染级别，含裂解液清除过滤器，25次

• 产品编号：K210017      品牌：invitrogen       原厂货号：K210017
• 产品分类：DNA和RNA纯化 > DNA纯化 > 质粒DNA纯化
• 应用分类：核酸分离纯化与分析 > DNA纯化 > 质粒 DNA 纯化

其它组分：

描述：

PureLink™ HiPure Filter Plasmid Kits are designed to isolate plasmid DNA of the highest purity from 25 to 500 mls of bacterial cultures (Table 1). The PureLink™ HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of the bacterial lysate with anion-exchange resin purification of plasmid DNA, in a single unit. The resulting plasmid DNA is of a quality equivalent to that obtained by purification with two passes through cesium chloride gradients - the most rigorous method for DNA purification. Purify high quality DNA in less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins. Additionally, the elimination of phenol, chloroform, ethidium bromide, and cesium chloride from the protocol minimizes exposure to, and disposal of hazardous materials.

The PureLink™ HiPure Plasmid Filter Kits provide:
• Simple protocols - bacterial lysate 查看更多clarification without centrifugation
• Fast results - save time by purifying plasmid DNA in less than two hours
• High yields - up to 200 µg for midipreps and up to 850 µg for maxipreps (Table 1)
• Reliable performance - the high-quality plasmid DNA purified with these kits is suitable for a wide variety of applications



How It Works

The addition of a HiPure Filter Column to the PureLink™ HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation (Figure 1). The lysate filtration cartridge is integrated into the DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin. A single column wash removes impurities such as RNA, proteins, metabolites, and other low-molecular-weight molecules, and the ultrapure plasmid DNA is eluted with a high-salt buffer. The DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation. The entire protocol can be completed in about two hours.

原厂资料：

注意事项：

The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.