The BacTiter-Glo™ Microbial Cell Viability Assay is a homogeneous method for determining the number of viable microbial cells in culture based on quantitation of the ATP present. ATP is an indicator of metabolically active cells. The homogeneous assay procedure involves adding a single reagent (BacTiter-Glo™ Reagent) directly to bacterial cells cultured in medium and measuring luminescence. The homogeneous format reduces pipetting errors that may be introduced during the multiple steps required by other methods of ATP measurement. The formulation of the reagent supports bacterial cell lysis and generation of a luminescent signal in a homogeneous "add-mix-measure" format. The luminescent signal is proportional to the amount of ATP present, which is directly proportional to the number of viable cells in culture. The assay relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase) and a proprietary buffer formulation for extracting ATP from bacteria. The assay has been shown to detect a variety of bacteria and fungi.
Features - Benefits
Simplify Microbial Detection: The "add-mix-measure" format reduces the number of handling steps to fewer than that required for similar ATP assays, with no separate lysis step, and no injectors required, allowing easy automation.
Get Results Quickly: Data can be recorded in 5 minutes or less after adding reagent and mixing. Superior sensitivity allows you to detect growth or toxicity quickly after inoculation.
Increase Your Sensitivity: Measure ATP from as few as 10 bacterial cells, 1,000-fold more sensitive than absorbance (O.D.) readings.
Choose Your Format: Can be used with various multiwell-plate or single-use formats. Data can be recorded by luminometer or CCD camera.
Process Plates Consecutively: The "glow-type" luminescent signal is stable, with a half-life generally over 30 minutes.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Applications
Examining antimicrobial compound activity.
Screening antimicrobial compounds.
Examining bacterial growth with extended sensitivity and range.
Rapid bacterial quantitation.
Rapid ATP detection.
For more information, see the Protocols & Applications Guide.
Notes
Using 100μl of BacTiter-Glo™ Reagent per assay in a 96-well format, Cat.# G8230 provides sufficient reagents to perform 100 assays; Cat.# G8231 and G8232, 1,000 assays; Cat.# G8233, 10,000 assays. Using 25μl of BacTiter-Glo™ Reagent per assay in a 384-well format, Cat.# G8230 provides sufficient reagents to perform 400 assays; Cat.# G8231 and G8232, 4,000 assays; Cat.# G8233, 40,000 assays. rATP (Cat.# P1132) will need to be purchased separately for performing a standard curve.
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