保存缓冲液组分：25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT和50% (v/v)甘油。
10X 反应缓冲液：500 mM Tris-HCl (pH 8.0, 25°C), 50 mM MgCl2, 10 mM DTT
描述：
Thermo Scientific Klenow Fragment is the large fragment ofDNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.
Activein restriction enzyme, PCR, RT, andT4 DNA Ligasebuffers
Applications
DNA blunting by fill-in 3'-overhangs
Random-primed DNA labeling
Labeling by fill-in 5'-overhangs of dsDNA
DNA sequencing by the Sanger method
Site-specific mutagenesis of DNA with synthetic oligonucleotides
Second strand synthesis of cDNA
原厂资料：
Thermo Scientific Klenow Fragment is the large fragment ofDNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.
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