The EnzChek® Protease Assay Kit, green fluorescence, is a fast, simple, and direct fluorescence-based assay for detecting metallo-, serine, acid, and sulfhydryl proteases. The accompanying increase in fluorescence, which can be measured with fluorescence microplate reader, is proportional to protease activity.
• Generalized mechanism allows for measurement of a variety of proteases • Can be used to continuously measure protease kinetics • Excitation/emission of 505/513 nm allows use of standard fluorescein (FITC) filter settings
This EnzChek® Protease Assay Kit contains a casein derivative that has been extensively labeled with the pH-insensitive, green-fluorescent BODIPY® FL dye, which results in a quenching of the fluorescent dye. Protease-catalyzed hydrolysis releases the highly-fluorescent BODIPY® FL dye-labeled peptides, allowing for quantitative detection of protease activity in solution. The green-fluorescent BODIPY® FL dye has excitation and emission spectra similar to those of fluorescein.
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For Research Use Only. Not for use in diagnostic procedures.