Caldesmon-1 (D5C8D) XP? Rabbit mAb

• 产品编号：CST-12503P      品牌：Cell Signaling Technology       原厂货号：12503P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caldesmon-1 (D5C8D) XP® Rabbit mAb可以识别内源性总的Caldesmon-1 蛋白。基于序列同源性，该抗体预期与平滑肌和非肌肉亚型有交叉反应。caldesmon-1是一个肌动蛋白丝稳定蛋白，设计了调控细胞收缩。磷酸化以及在钙存在时的钙调蛋白会减弱caldesmon-1与actin的结合。caldesmon-1由单基因编码，会被剪切形成一个广泛表达的低分子量亚型和一个平滑肌特异性的高分子亚型（1,2）。caldesmon-1会在细胞周期依赖性激酶cdc2和Erk1/2 MAP激酶的存在下被磷酸化，这两种磷酸化都会阻止caldesmon-1的活性 (3-5).cdc2磷酸化caldesmon-1 是细胞通过有丝分裂进行传代所必须的（6）。Erk1/2的磷酸化对于调控平滑肌收缩非常重要（7）。caldesmon-1 的活性可能在伪足形成，黏附复合物与基质金属蛋白酶的结合，侵润和转移等过程中发挥了左右（5）。

1. Hayashi, K. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 12122-12126.
2. Humphrey, M.B. et al. (1992) Gene 112, 197-204.
3. Yamashiro, S. et al. (1991) Nature 349, 169-172.
4. Mak, A.S. et al. (1991) J. Biol. Chem. 266, 6678-6681.
5. Hai, C.M. and Gu, Z. (2006) Eur. J. Cell Biol. 85, 305-309.
6. Yamashiro, S. et al. (2001) Mol. Biol. Cell 12, 239-250.
7. Hedges, J.C. et al. (2000) Am. J. Physiol. Cell Physiol. 278, C718-C7126.

原厂资料：

Specificity / Sensitivity

Caldesmon-1 (D5C8D) XP® Rabbit mAb recognizes endogenous levels of total caldesmon-1 protein. Based on sequence homology, the antibody is expected to cross-react with both the smooth muscle and nonmuscle isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caldesmon-1 protein.

Background

Caldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5).

1. Hayashi, K. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 12122-12126.
2. Humphrey, M.B. et al. (1992) Gene 112, 197-204.
3. Yamashiro, S. et al. (1991) Nature 349, 169-172.
4. Mak, A.S. et al. (1991) J. Biol. Chem. 266, 6678-6681.
5. Hai, C.M. and Gu, Z. (2006) Eur. J. Cell Biol. 85, 305-309.
6. Yamashiro, S. et al. (2001) Mol. Biol. Cell 12, 239-250.
7. Hedges, J.C. et al. (2000) Am. J. Physiol. Cell Physiol. 278, C718-C7126.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.