LIS1 Antibody

• 产品编号：CST-12453S      品牌：CST       原厂货号：12453S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LIS1 Antibody兔多抗能够检测内源性的LIS1总蛋白。该多克隆抗体是由合成的人源LIS1蛋白甘氨酸(298位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。LIS1是一种细胞骨架相互作用的蛋白质，它包含一个N-末端的二聚化结构域和C-末端与动力蛋白的马达结构域相互作用的β-螺旋域（1-3）。研究表明LIS1基因的突变导致无脑回畸形 ，一种大脑发育存在严重缺陷的疾病（4）。 LIS1在大脑皮质迁移和发育中也起着至关重要的作用（5）。 LIS1的活性对于兴奋性突触在发育中的中间神经元树突中以微管依赖性的方式进行的逆行易位是必需的（6）。

1. Sasaki, S. et al. (2000) Neuron 28, 681-96.
2. Tai, C.Y. et al. (2002) J Cell Biol 156, 959-68.
3. Tarricone, C. et al. (2004) Neuron 44, 809-21.
4. Reiner, O. et al. (1993) Nature 364, 717-21.
5. Wynshaw-Boris, A. (2007) Clin Genet 72, 296-304.
6. Kawabata, I. et al. (2012) Nat Commun 3, 722.

原厂资料：

Specificity / Sensitivity

LIS1 Antibody recognizes endogenous levels of total LIS1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly298 of human LIS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LIS1 is a cytoskeleton-interacting protein that contains an N-terminal dimerization domain and a C-terminal β-propeller domain that interacts with the motor domain of dynein (1-3). Research studies have shown that mutations in the LIS1 gene are involved in lissencephaly, a disease characterized by severe defects in brain development (4). LIS1 also plays a critical role in cortical migration and development in the brain (5). LIS1 activity is required for retrograde translocation of excitatory synapses in developing interneuron dendrites in a microtubule-dependent fashion (6).

1. Sasaki, S. et al. (2000) Neuron 28, 681-96.
2. Tai, C.Y. et al. (2002) J Cell Biol 156, 959-68.
3. Tarricone, C. et al. (2004) Neuron 44, 809-21.
4. Reiner, O. et al. (1993) Nature 364, 717-21.
5. Wynshaw-Boris, A. (2007) Clin Genet 72, 296-304.
6. Kawabata, I. et al. (2012) Nat Commun 3, 722.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.