NRF1 Antibody

• 产品编号：CST-12381S      品牌：CST       原厂货号：12381S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NRF1 Antibody能够检测内源性NRF1总蛋白水平。该多克隆抗体是由合成的人源NRF1蛋白亮氨酸(167位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。核呼吸因子1（NRF1）是编码细胞色素c的基因的转录激活因子（1）。后来发现在核调控线粒体功能中发挥作用（1）。 PGC-1诱导NRF1和Nrf2的表达（2）。NRF1，与共激活因子PGC-1一起刺激线粒体转录因子A的启动子，调节线粒体遗传和功能（2）。最近，发现哺乳动物NRF1/2/3的直系同源基因秀丽隐杆线虫SKN-1在胁迫耐性和和长寿方面的作用（3）。在线虫中，当TORC1和TORC2被抑制时， 需要SKN-1/NRF介导抗应激能力和延长寿命（4）。

1. Virbasius, C.A. et al. (1993) Genes Dev 7, 2431-45.
2. Wu, Z. et al. (1999) Cell 98, 115-24.

原厂资料：

Specificity / Sensitivity

NRF1 Antibody recognizes endogenous levels of total NRF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu167 of human NRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Nuclear respiratory factor 1 (NRF1) was identified as a transcription activator for the gene encoding cytochrome c (1). It was later found to play a role in the nuclear control of mitochondrial function (1). PGC-1 induces the expression of NRF1 and NRF2 (2). NRF1, along with the coactivator PGC-1, stimulates the promoter of mitochondrial transcription factor A, which regulates mitochondrial biogenensis and function (2). Recently, C. elegans SKN-1, the ortholog of mammalian NRF1/2/3, was implicated in stress resistance and longevity (3). In C. elegans, SKN-1/NRF is required to mediate the stress resistance and extended life span when either TORC1 or TORC2 is inhibited (4).

1. Virbasius, C.A. et al. (1993) Genes Dev 7, 2431-45.
2. Wu, Z. et al. (1999) Cell 98, 115-24.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.