LLGL1 (D2B5A) Rabbit mAb

• 产品编号：CST-12159S      品牌：CST       原厂货号：12159S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LLGL1 (D2B5A) Rabbit mAb可以识别内源性总的LLGL1蛋白。抗体与LLGL2没有交叉反应。单克隆抗体由合成肽段免疫动物产生，该肽段与人LLGL1蛋白的Leu757及其邻近氨基酸残基序列一致。果蝇中，lethal giant larvae (lgl), discs large (dlg), and scribble (scrib)是抑癌基因。它们的功能缺失会导致幼虫大脑的肿瘤性增生和成虫上皮细胞出现细胞骨架和细胞极化受损的标记（1,2）。果蝇lgl蛋白的人类同源物，ethal giant larvae protein homolog 1 
 (LLGL1),是一个细胞骨架蛋白调控了细胞器生成，细胞迁移和细胞极化（3）。果蝇中LLGL1表达下降与细胞增生和恶性转化相关（4-6）。哺乳动物上皮细胞中，LLGL1从细胞质移动到细胞-细胞连接处，以形成并保持计划的特征（7）。LLGL1也在上皮细胞与PAR6 和aPKC连接的形成过程中发挥作用，后者认为可以磷酸化LLGL1的Ser663，因此限制它定位到细胞的基底外侧（8）。LLGL1也在细胞发育和分化过程中发挥了左右，果蝇的lgl被认为会控制祖细胞的自我更新和分化（9）。最近对小鼠的研究提示哺乳动物LLGL1同源物没有肿瘤抑制样功能，LLGL2,则被认为对于胚胎发育时期滋养层细胞合理的极化浸润和有效的形态发生是必须的（10）。

1. Agrawal, N. et al. (1995) Dev Biol 172, 218-29.
2. Massimi, P. et al. (2008) Exp Cell Res 314, 3306-17.
3. Strand, D. et al. (1995) Oncogene 11, 291-301.
4. Schimanski, C.C. et al. (2005) Oncogene 24, 3100-9.
5. Kuphal, S. et al. (2006) Oncogene 25, 103-10.
6. Lu, X. et al. (2009) Clin Cancer Res 15, 3287-96.
7. Müsch, A. et al. (2002) Mol Biol Cell 13, 158-68.
8. Yamanaka, T. et al. (2003) Curr Biol 13, 734-43.
9. Mechler, B.M. et al. (1985) EMBO J 4, 1551-7.
10. Sripathy, S. et al. (2011) Mol Cell Biol 31, 2920-33.

原厂资料：

Specificity / Sensitivity

LLGL1 (D2B5A) Rabbit mAb recognizes endogenous levels of total LLGL1 protein. This antibody does not cross-react with LLGL2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu757 of human LLGL1 protein.

Background

In Drosophila, lethal giant larvae (lgl), discs large (dlg), and scribble (scrib) act as tumor suppressor genes. Their loss of function in flies causes neoplastic overgrowth of larval brain tissue and imaginal epithelial cells hallmarked by disruption of the cytoskeletal network and cellular polarity (1,2). The human homolog of the Drosophila lgl protein, lethal giant larvae protein homolog 1 
 (LLGL1), is a cytoskeletal protein implicated in regulating cellular organization, migration, and cell polarity (3). As in Drosophila, decreased expression of LLGL1 correlates with an increased incidence of cellular overgrowth and malignant transformation (4-6). In mammalian epithelial cells, LLGL1 redistributes from the cytoplasm to regions of cell-cell contact, allowing the establishment and maintainence of a polarized morphology (7). LLGL1 also plays a role in the formation of epithelial junctions via its direct interactions with PAR6 and aPKC, the latter of which has been shown to phosphorylate LLGL1 at Ser663, thus restricting its localization to the basolateral region of the cell (8). LLGL1 may also play an additional, unrealized role in cellular development and differentiation as indicated by the fact that Drosophila lgl has been implicated in controlling self-renewal and differentiation of progenitor cells (9). Recent studies in mice have suggested that a mammalian LLGL1 homolog that does not have tumor suppressor-like acitvity, LLGL2, is required for proper polarized invasion of trophoblasts and efficient branching morphogenesis during placental development (10).

1. Agrawal, N. et al. (1995) Dev Biol 172, 218-29.
2. Massimi, P. et al. (2008) Exp Cell Res 314, 3306-17.
3. Strand, D. et al. (1995) Oncogene 11, 291-301.
4. Schimanski, C.C. et al. (2005) Oncogene 24, 3100-9.
5. Kuphal, S. et al. (2006) Oncogene 25, 103-10.
6. Lu, X. et al. (2009) Clin Cancer Res 15, 3287-96.
7. Müsch, A. et al. (2002) Mol Biol Cell 13, 158-68.
8. Yamanaka, T. et al. (2003) Curr Biol 13, 734-43.
9. Mechler, B.M. et al. (1985) EMBO J 4, 1551-7.
10. Sripathy, S. et al. (2011) Mol Cell Biol 31, 2920-33.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting  1:1000
Immunofluorescence (IF-IC)  1:50