Description: The human sTNF-R (60kDa) Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of soluble human 60 kDa Tumor Necrosis Factor-Receptor. The human sTNF-R (60kDa) Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.
TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines. Based on gel filtration experiments the receptor appears to be a complex of different proteins with a molecular weight of 350 kDa. In a variety of cell lines two different types of TNF receptors with 75-80 and 55-60 kDa respectively have been identified. The cDNAs encoding the two different TNF receptors have been cloned. The exact mechanism of signal transduction after binding of TNF to the receptor is still unclear. The extracellular fragment of the 60 kDa TNF receptor, with a molecular mass of about 30 kDa was purified, partially sequenced, and the respective cDNA was cloned. This TNF binding protein is liberated from the intact molecule by proteolytic cleavage and comprises most of the extracellular portion of the receptor, including all three N-glycosylation sites.
The present assay provides a simple, rapid, and highly sensitive method for the determination of soluble TNF-R (60 kDa) levels in body fluids or cell culture supernatants. This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (60 kDa) in various neoplastic and inflammatory diseases.
Components
Aluminium pouch(es) with a Microwell Plate coated with Monoclonal Antibody (murine) to human sTNF-R (60 kDa) and HRP-Conjugate (anti-sTNF-R (60 kDa) monoclonal (murine) antibody), lyophilized
Aluminium pouch(es) with a human sTNF-R (60kDa) Standard curve (coloured)
Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Assay Buffer Concentrate 20x (Use when an external predilution of the samples is needed)
Stop Solution (1M Phosphoric acid)
Adhesive Plate Covers