OPA1 Pure 18/OPA-1 150ug

Three major GTP-binding protein families include trimeric and low molecular weight G-proteins, as well as a family of large proteins

homologous to dynamin. The dynamin family contains proteins with diverse structure and function, but highly homologous N-terminal

GTPase domains. A subgroup of the dynamin G-protein-binding family includes the mitochondrial proteins Drp1/Dnm1, Mgm1, and OPA1.

The latter protein is mutated in dominant optic atrophy, a disease that involves loss of visual acuity and atrophy of the optic nerve. OPA1 is

expressed in heart, brain, liver, and kidney. The sequence of OPA1 includes an N-terminal region that contains a mitochondrial targeting

domain and three GTP-binding motifs. The overexpression of OPA1 in Cos-7 cells shows co-localization with cytochrome c in mitochondria,

and leads to alterations in mitochondrial morphology from a characteristic tubuluar shape to a vesicular pattern. Thus, OPA1 may have roles in

mitochondrial biogenesis that are critical for normal cell function.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.