Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of
multicellular organisms. Apoptotic programs in virus-infected cells regulate viral replication and pathogenesis. However, viruses, such as
human adenovirus, have evolved methods of circumventing these programs. The adenovirus E1B encodes a 19 kDa protein (E1B 19K) that,
similar to Bcl-2, suppresses apoptosis via interactions with intracellular proteins. Btf (Bcl-2 associated Transcription Factor) interacts with
E1B 19K, Bcl-2, and Bcl-xL. Two forms of Btf, Btf [L] and Btf [S], differ due to a 49 amino acid deletion in the C-terminal region of Btf [S].
Both forms contain putative basic zipper-like (bZIP) and Myb-like DNA-binding domains. In vitro, Btf binds DNA and represses
transcriptional activity. Cotransfection of E1B 19K, Bcl-2, Bcl-xL, and Btf results in the cytoplasmic sequestration of Btf and inhibition of its
transcriptional repression activity. Overexpression of Btf induces apoptosis, which is inhibited by E1B 19K. Thus, Btf is an important
death-promoting transcriptional factor, which is regulated by anti-apoptotic members of the Bcl-2 family.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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