Casein Kinase II Alp/Alp Pure 31 150ug

Casein kinase II (CKII, CK2) is a pleiotropic, ubiquitous, and constitutively active Ser/Thr kinase that utilizes both ATP and GTP as

phosphoryl donors to catalyze the phosphorylation of numerous protein substrates. It has been implicated in DNA replication, control of

metabolism, and regulation of transcription and translation. CKII is a messenger independent kinase due to the fact that its activity is not

affected by factors that regulate other protein kinases (e.g. cAMP, Ca2+, diacylglycerol). It is composed of two catalytic (α and α') subunits

and two regulatory (β) subunits. The β subunits stabilize the holoenzyme and enhance the activity of the α subunits. Although the enzyme

exists as a tetramer, the free α/α' subunits are catalytically active by themselves and are present in cells under some conditions. The α and α'

subunits (85% homologous) are encoded by distinct genes and have been detected in multiple species including human, bovine, and avian.

CKII is found primarily in the nucleus in dividing cells, but can be detected in the cytoplasm in quiescent cells. Thus, CKII activity is required

for cell cycle progression and other essential signal transduction events.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.