Rac1 Pure 102/Rac1 50ug

Rac1 is a member of the expanding family of Ras-related GTPase proteins. These

small proteins have the ability to switch between two different conformational

states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase

activity is increased by specific activator proteins named GAPs. Rac1, contains

the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma

membrane. Growth factor stimulation of cells results in a dramatic reorganization

of the actin filament network and membrane ruffling, processes for which Rac1 is

essential. Constitutive expression of Rac1 leads to cell transformation. This

process is mediated by the Rac1-dependent activation of the p65PAK

serine/threonine kinase. IQGAP1 has been identified as a GTPase activating

protein specific for Rac1.

Testing in native ELISA with recombinant proteins to CDC42 and Rho have shown

that the antibody to Rac1 (cat No 610650/1) recognizes CDC42 and Rho. Due to

sequence homology among various small GTPase familyl members, potential

reactivity could be observed with other family members.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.