BD Pharmingen™ Red FP Vector - Endoplasmic Reticulum is a mammalian expression vector that encodes a fusion of the red fluorescent
protein (FP) from Discosoma sp. with an endoplasmic reticulum (ER) targeting sequence fused to its 5’- end and an ER retention sequence,
KDEL, fused to its 3'-end. In order to increase the translation efficiency in mammalian cells, a Kozak consensus translation initiation site has
been introduced to the 5’-end of the red FP open reading frame, and its sequence has been optimized with human codons. The red FP fusion is
expressed under the control of the immediate early promoter of cytomegalovirus (P CMV ie), and its sequence is followed by downstream
simian virus 40 (SV40) polyadenylation signals. The vector contains a neomycin resistance gene controlled by the SV40 promoter (P SV40 e)
that allows selection of stably transfected eukaryotic cells using G418. An additional bacterial (Kan R) promoter drives the expression of the
same gene encoding for kanamycin resistance in E. coli.