Phosflow Lyse/Fix Buffer 5X 250mL

包装：

250mL

运保温度:

Store undiluted at room temperature.

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描述：

BD Phosflow™ Lyse/Fix Buffer is intended to be used for the lysing and fixing of whole blood for use with intracellular flow cytometry. For

example, after cell stimulation with kinase activators, such as phorbol esters, the BD Phosflow™ Lyse/Fix buffer can be used for lysing red

blood cells and for fixing leukocytes in one step. This allows investigators to rapidly inactivate kinases, phosphatases and/or proteases, so that

the in vivo phosphorylation state of the cell can be examined. The BD Phosflow™ Lyse/Fix Buffer has been reported to preserve the light

scattering properties of the cell and may be used on human and mouse whole blood. Whole blood lysis has been shown to be as effective as

density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis. In many cases, phospho-specific antibodies may

be used simultaneously with the staining of cell surface markers (e.g CD markers). Not all cell surface markers, however, are compatible with

the BD Phosflow™ Lyse/Fix Buffer. For a current listing of compatible cell surface markers for use with BD Phosflow™ Lyse/Fix Buffer,

investigators are encouraged to visit the following website: http://www.bdbiosciences.com/docs/Validated_Cell_Surface_Markers.xls.

注意事项：

Store undiluted at room temperature.