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Hu/NHP TNF PE MAb11 50Tst

  • 产品编号:BD-557068      品牌:BD-Pharmingen       原厂货号:557068
  • 产品分类:抗体 > 一抗 > 染料标记抗体
  • 应用分类:
 
包装: 50Tst
运保温度: Store undiluted at 4°C
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描述:

Clone MAb11 reacts with the human form of tumor necrosis factor (TNF, formerly known as TNF-α) detectable in the cytoplasm of a major
subset of activated peripheral blood T lymphocytes. Using standard procedures for detection of intracellular proteins, clone MAb11 also
cross-reacts with activated peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following
five-hour treatment with phorbol myristic acetate (PMA) and Ca++ Ionophore (A23187) in the presence of monensin. The staining is
restricted to CD3+ T cells and is similar to that observed with peripheral blood T lymphocytes from normal human donors.


注意事项:

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).
2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
5.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.


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