PhosphoWorks™ Colorimetric MESG Phosphate Assay Kit *UV absorption*

• 产品编号：AAT-21659      品牌：AAT       原厂货号：21659
• 产品分类：生化和细胞分析 > 生化分析试剂盒 > 磷酸盐&焦磷酸盐分析试剂盒
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描述：

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red. This feature has been used to develop our convenient MESG phosphate assay kit. Our kit provides all the essential reagents including MESG, phosphorylase and reaction buffer. The MESG substrate gives an absorbance increase at 360 nm on phosphorylysis at pH 6.5-8.5, and at pH 7.6 the change in extinction coefficient is 11,000 M-1cm-1. The assay is shown to quantitate phosphate in solution at concentrations at least down to 2 µM. It can be used to measure the kinetics of phosphate release from phosphatases (such as GTPases and ATPases) by coupling the two enzymatic reactions.

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