Phosphatase-catalyzed hydrolysis of Sun Red phosphate (SRP) yields the Sun Red fluorophore that can be excited with the 633 nm laser with emission of ~660 nm. Although Sun Red is readily excited at 633 nm with red emission of ~660 nm, SRP has very minimal absorption at 633 nm without red emission, making SRP one of the most sensitive NIR phosphatase sensors. Please do not use DMSO to make stock solution since it significantly increases assay background.
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