GoScript? Reverse Transcription System

• 产品编号：A5001      品牌：promega       原厂货号：A5001
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > RT-PCR（Endpoint）
• 应用分类：PCR > 反转录 > 反转录酶及试剂盒

其它组分：

描述：

原厂资料：

The GoScript™ Reverse Transcription System is a convenient kit that includes a reverse transcriptase and an optimized set of reagents for efficient synthesis of first-strand cDNA optimized in preparation for PCR amplification. The components of the GoScript™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with either total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and reverse transcriptase provided in the GoScript™ Reverse Transcription System enable robust, full-length cDNA synthesis for reproducible analysis of rare or long messages. These conditions were developed for cDNA synthesis or for easy transition to gene-specific target amplification. Up to 5μl of the reverse transcription reaction can be directly amplified using Taq DNA polymerase in a 25µl PCR. GoScript™ Reverse Transcriptase is qualified for use in two-step RT-qPCR, using GoTaq® qPCR and Plexor® qPCR Systems. In two-step RT-qPCR using GoTaq® qPCR Master Mix, samples of the GoScript™ Reverse Transcription System can be added directly, up to 20% v/v.

Product Size Cat.#
GoScript™ Reverse Transcription System 100 reactions A5001

General Laboratory Precautions
• Use designated work areas and pipettors for pre- and post-amplification steps.
This precaution is intended to minimize the potential for cross-contamination
between samples and prevent carryover of nucleic acid (DNA and RNA) from
one experiment to the next.
• Wear gloves and change them often.
• Prevent contamination by using barrier or positive displacement pipette tips.
• Use sterile, nuclease-free thin-walled reaction tubes.
• The GoScript™ Reverse Transcriptase, GoScript™ 5X Reaction Buffer and PCR
Nucleotide Mix should be kept chilled before use. Thaw on ice; do not thaw by
heating in a warming block.
Note: The characteristics of the products of the reverse transcriptase reaction run in
the 37–55°C temperature range may vary, depending on the RNA template and the
method of analysis of the products.

Each system contains sufficient reagents for 100 first-strand cDNA synthesis reactions
of 20μl each.
• 100μl GoScript™ Reverse Transcriptase
• 600μl GoScript™ 5X Reaction Buffer
• 1.2ml MgCl2 (25mM)
• 200μl PCR Nucleotide Mix
• 50μg Oligo(dT)15 Primer
• 50μg Random Primers
• 1.25ml Nuclease-Free Water
• 2,500u Recombinant RNasin® Ribonuclease Inhibitor

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors. GoScript™ Reverse Transcriptase is qualified for use in qPCR, including GoTaq® qPCR and Plexor® RT-qPCR systems.

Features - Benefits

• Ultra-Active: Save money on every reaction.
• Sensitive: Detect rare transcripts.
• Processive: Transcribe long messages.
• Resilient: Synthesize cDNA in the presence of strong inhibitors.

Applications

• RT-qPCR.
• RT-PCR.

注意事项：