Wizard? Plus SV Minipreps DNA Purification System

产品编号：A1460 品牌：promega 原厂货号：A1460
产品分类：DNA和RNA纯化 > DNA纯化 > 质粒DNA纯化
应用分类：核酸分离纯化与分析 > DNA纯化 > 质粒 DNA 纯化

包装：

250 preps

运保温度:

15–30°C

到货周期：

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其它组分：

Wizard® SV Minicolumns【子货号：A129B，包装：1 x 250/pk，，运保温度：15–30°C】

Collection Tubes【子货号：A130B，包装：1 x 250/pk，，运保温度：15–30°C】

Column Wash Solution (CWA)【子货号：A131C，包装：1 x 100ml，，运保温度：15–30°C】

Alkaline Protease Solution【子货号：A144B，包装：1 x 3ml，，运保温度：15–30°C】

Neutralization Solution (NSB)【子货号：A148C，包装：1 x 100ml，，运保温度：15–30°C】

Cell Resuspension Solution (CRA)【子货号：A711N，包装：1 x 75ml，，运保温度：15–30°C】

Cell Lysis Solution (CLA)【子货号：A712N，包装：1 x 75ml，，运保温度：15–30°C】

Nuclease-Free Water【子货号：P119C，包装：1 x 25ml，，运保温度：15–30°C】

描述：

说明

Wizard^® Plus SV Minipreps DNA Purification System(Wizard^® Plus SV 质粒小量纯化系统) 以柱膜为基础，可快速抽提质粒DNA，操作方法简单、可靠。根据处理样品的数目，整个过程可在45 分钟内完成。使用该系统可从1-10ml 过夜培养的E.coli 培养液中纯化质粒DNA。纯化的质粒DNA 可直接用于BigDye^®终止法自动化荧光测序及其它分子生物学应用，也可加入核酸酶抑制剂( 如重组RNasin^® ,Cat.#N2511) 后用于体外转录反应。

特点

. 高效率：在45 分钟内纯化20 个样品。

• 高性能：可获得1-20μg 满足多种应用的高质量质粒DNA。

• 安全方便：不需苯酚抽提或乙醇沉淀。

• 灵活：可选择离心或真空模式纯化质粒DNA。

• 质量可靠：碱性蛋白酶处理提高质粒纯化质量。

• 结果可靠：每批产品用pGEM^®-3Zf(+) 载体通过BigDye^® 终止测序法分析，可读取大于500 个碱基，准确率超过98%。

原厂资料：

Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.

The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Features - Benefits

Improved Productivity: 20 minipreps processed in less than 45 minutes.
High Performance: 1–20μg of high-quality plasmid DNA, enough for multiple applications.
Safety and Convenience: No phenol extractions or precipitations required.
Flexibility: Choice of spin (microcentrifuge) or vacuum purification formats.
Consistent Quality: Alkaline protease step improves plasmid quality.
Confidence in Results: Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing.

Applications

For more information, see the Protocols & Applications Guide.

注意事项：

Do not exchange or replace components of the Wizard® Plus SV Minipreps
DNA Purification System with components from any other Wizard® Plus System.
Components from the Wizard® Plus and Wizard® Plus SV Systems are not
interchangeable.

Materials to Be Supplied by the User
(Solution compositions are provided in Section 6.)
• LB agar plates containing antibiotic
• LB medium containing antibiotic
• ethanol (95%)
• microcentrifuge capable of 14,000 × g
• sterile 1.5ml microcentrifuge tubes
• centrifuge capable of 10,000 × g

Prior to using a new Wizard® Plus SV Minipreps DNA Purification System, dilute
the provided Column Wash Solution (CWA) as follows:
Add 7ml of 95% ethanol for a final volume of 11ml for the 10-prep system
(Cat.# A1270).
Add 35ml of 95% ethanol for a final volume of 55ml for the 50-prep system
(Cat.# A1330 and A1340).
Add 170ml of 95% ethanol for a final volume of 270ml for the 250-prep system
(Cat.# A1460 and A1470)