Recombinant human LC3B protein corresponding to amino acids 2–120
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/ml
Purification
Protein A
Storage buffer
PBS
Preservative
0.09% sodium azide
Storage Conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
ChIP assay (ChIP)
1 ul
Flow Cytometry (Flow)
3-5 ug x 10^6 cells
Immunocytochemistry (ICC)
1-3 ug/ml
Immunofluorescence (IF)
1-3 ug/ml
Immunohistochemistry (Paraffin) (IHC (P))
1:20-1:200
Western Blot (WB)
1-5 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
LC3B (Autophagy Marker Light Chain 3B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). The product of this gene is associated with microtubule assembly and important is in neurogenesis. Recent studies indicates that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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