Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Tested Applications
Dilution *
Flow Cytometry (Flow)
3-5 µg x 10^6 cells
Immunocytochemistry (ICC)
2 µg/ml
Immunofluorescence (IF)
2 µg/ml
Western Blot (WB)
0.05-0.5 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This antibody is predicted to react with Rhesus monkey and porcine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Background/Target Information
Monocyte chemotactic protein-1 (MCP-1) also known as CCL2 has a predicted molecular weight of ~11kDa; however, it may run at a higher molecular weight due to post translational modifications such as glycosylation. MCP-1 is a member of cytokine family and plays an important role in immunoregulation and inflammatory processes. MCP-1 is indicated in the etiology of several immune disorders. MCP-1 is also linked with tumor progression and related angiogenesis. MCP-1 displays selective chemotactic activity towards monocytes and basophils but not neutrophils or eosinophils. MCP-1 binds to chemokine receptors CCR2 and CCR4.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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