CD CHO 培养基是化学成分确定的培养基,适合中国仓鼠卵巢 (CHO) 细胞的培养以及悬浮培养中重组蛋白质的表达。 CD CHO 培养基不含动物、植物或合成来源的蛋白质或肽成分。 另外,配方中不含成分不确定的裂解物或水解物。 CD CHO 培养基不含二氢叶酸还原酶 (DHFR) 扩增系统中的次黄嘌呤和胸腺嘧啶;对于其它应用,在使用前添加 10 ml/L HT Supplement(目录号: 11067-030)。 配方中不含 L-谷氨酰胺以增加稳定性;使用前每 1,000 ml 培養基添加 40 ml 200 mM L-谷氨酰胺。 不含谷氨酰胺、次黄嘌呤、胸腺嘧啶或酚红。
原厂资料:
CD CHO Medium has been developed for the growth of Chinese Hamster Ovary (CHO) cells and expression of recombinant proteins in suspension culture. CD CHO is an animal origin-free (AOF), chemically defined medium that contains no proteins, hydrolysates, or components of undefined
composition. CD CHO is formulated without hypoxanthine and thymidine for use in dihydrofolate reductase (DHFR) amplified systems, and without phenol red to minimize estrogen-like effects of phenol red.