亮抑酶肽Leupeptin Hemisulfate

• 产品编号：0215155305      品牌：MP       原厂货号：0215155305
• 产品分类：克隆 > DNA/RNA修饰酶 > RNA酶抑制剂
• 应用分类：暂定分类 > 标签蛋白的分离

描述：

(Acetyl-Leu-Leu-Arg-al)
Hemisulfate
Inhibitor for trypsin, plasmin, papain, and cathepsin B.
Ref: Kondo, S., et al., Chem. Pharm. Bull., 17: 1869, (1969).

Description: A modified tripeptide reversible protease inhibitor of trypsin-like proteases and some cysteine proteases¹² including endoproteinase Lys-C, kallikrein, papain, thrombin, cathepsin B⁶, cathepsins H and L¹⁴, trypsin, calpain¹³, trypsin⁸ and plasmin. Effective concentration is typically 10-100 uM. Little to no inhibition is seen against pepsin, cathepsins A and D and alpha-chymotrypsin. When adjusted for molarity, all three salt forms are equally effective; however, the hydrochloride salt is usually less invasive in biological settings.

Leupeptin, because of its aldehyde group, may act as a reducing agent and therefore interfere in protein determinations such as Lowry and, to a lesser extent, Bradford.

亮抑酶肽；亮抑蛋白酶肽

用途：蛋白酶抑制剂

破碎细胞提取蛋白质的同时可释放出蛋白酶，这些蛋白酶需要迅速的被抑制以保持蛋白质不被降解。在蛋白质提取过程中，需要加入蛋白酶抑制剂以防止蛋白水解。

可在不同程度上抑制胰蛋白酶、纤溶酶、激肽释放酶、木瓜蛋白酶及组织蛋白酶；抑制丝氨酸和半胱氨酸蛋白酶。

用途：亮抑肽酶（LEUPEPTIN）是一种可逆的，半胱氨酸和丝氨酸蛋白酶抑制剂，可抑制木瓜蛋白酶（papain），胰蛋白酶（trypsin），血纤维蛋白溶酶（plasmin），组织蛋白酶A和B(cathepsin A和B)，凝血酶（thrombin）和激肽释放酶（kallikrein）

 溶液配制：贮存液：10mg/ml 溶于水，4℃稳定一周，-20℃稳定6个月；
工作浓度：0.5 μg/ml。
                  
 储存条件：-22~0℃

原厂资料：

注意事项：

Note: Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined by HPLC should take into account the three main peaks.¹¹ Majority of contaminating peptide is racemized leupeptin.

The primary mechanism of inactivation is racemization of the L-arginal; the D-arginal form is totally inactive. If the aldehyde is oxidized but retains its L-configuration, the resulting compound does have some inhibitory activity.