PLAT. QRT-PCR THERMOSCRIPT SYS(100 RXN)

• 产品编号：11731015      品牌：invitrogen       原厂货号：11731015
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qRT-PCR
• 应用分类：

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描述：

Platinum® Quantitative RT-PCR ThermoScript™ One-Step System (1) is ideal for real-time quantitation of RNA molecules from total or poly(A)⁺ RNA in a single step. ThermoScript™ RT, a cloned avian reverse transcriptase, has been engineered to have reduced RNase H activity and high thermal stability (up to 70°C) to copy RNA with secondary structure and improve specificity of priming. Specificity and amplification efficiency are further enhanced through the use of Platinum® Taq DNA polymerase (3). The system can be used to quantitate the amount of specific mRNA from small numbers of cells (Figure 1). The Platinum® Quantitative RT-PCR ThermoScript™ One-Step System:

• Is optimized to provide maximal performance with fluorescent detection chemistries employed in real-time quantitative PCR

• Provides reduced RNase H activity for greater first-strand cDNA yield

• Takes place sequentially in a single tube, under conditions optimal for both ThermoScript™ RT and

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• Platinum® Taq DNA polymerase

• Reduces reaction-to-reaction variability for improved precision in quantitative RT-PCR

• Detects as little as 10 molecules of RNA template

• Allows precise quantification of mRNA(s) from 0.1 pg to 1 µg of total RNA

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