Human IL-17 RA/IL-17 R Alexa Fluor 700 MAb (Clone 133617) (25 TESTS)
Human IL-17 RA/IL-17 R Alexa Fluor 700 MAb (Clone 133617) (25 TESTS)
产品编号：RD-FAB177N-025 品牌：R&D
原厂货号：FAB177N-025
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描述：
Species Reactivity
Human
Specificity
Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Source
Monoclonal Mouse IgG1 Clone # 133617
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-17 R
Leu33-Trp320
Accession # Q96F46
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB177N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol forStaining Membrane-associated Proteins.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17 RA/IL-17 R
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
References:
Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
Detects human IL-17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Source
Monoclonal Mouse IgG1 Clone # 133617
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-17 R
Leu33-Trp320
Accession # Q96F46
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL-17 R in K562 Human Cell Line by Flow Cytometry.K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB177N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol forStaining Membrane-associated Proteins.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17 RA/IL-17 R
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL-17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
References:
Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
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![Flow Cytometry IL-17 RA/IL-17 R Antibody (133617) [Alexa Fluor® 700]](https://resources.rndsystems.com/images/datasheets/antibody/IL17_R_FAB177N_Flow_Cytometry_13578.jpg)
