DyNAmo SYBR Green qPCR Kit with ROX

产品编号：F-400RL 品牌：Finnzymes 原厂货号：F-400RL
产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qPCR
应用分类：

包装：

500 x 20ul

运保温度:

-20 C

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描述：

The master mix in Thermo Scientific DyNAmo SYBR Green qPCR Kit contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.

In DyNAmo SYBR Green qPCR Kit with ROX passive reference dye the ROX passive reference dye is supplied with the kit in a separate tube. This kit is recommended for instruments that require ROX normalization.

Highlights
•Sensitivity: Optimized 2X master mix allows reproducible detection of low-copy number templates.
•Speed: More efficient amplification coupled with increased polymerase processivity results in earlier Cq's and shorter overall qPCR reaction times (see Figures 1 and 2). (Quantification cycle, Cq, is the cycle at which the fluorescence reaches the threshold level. The threshold level is set manually or calculated automatically.)
•Convenience: 2X master mix, one tube only. In F-400RL ROX passive reference dye is provided in a separate vial.
•Broad dynamic range: DyNAmo SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude.

Includes

DyNAmo SYBR Green qPCR Kit
• 2X Master Mix (containing modified Thermus brockianus DNA Polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP)

DyNAmo SYBR Green qPCR Kit with ROX passive reference dye
• 2X Master Mix (containing modified Thermus brockianus DNA Polymerase, SYBR Green I dye, optimized PCR buffer, 5 mM MgCl2, dNTP mix including dUTP)
• 50X ROX passive reference dye