Dynabeads® Antibody Coupling Kit allows easy coupling of an antibody of your choice to the surface of uniform, 2.8 µm superparamagnetic Dynabeads® M-270 Epoxy beads. Following immobilization of your antibody, the beads can then be used for experiments such as immunoassays, immunoprecipitation (IP), co-immunoprecipitation (co-IP) of protein complexes, co-IP of protein-nucleic acid complexes, as well as many other downstream applications. The Dynabeads® Epoxy beads in this kit do not contain Tween® detergent, and so are ideal for use in mass spectrometry (MS) analysis. In addition to Dynabeads®, the kit supplies all buffers needed for the coupling and washing steps. Note that the SB buffer supplied in the kit contains Tween® detergent, so the SB buffer will need to be replaced with standard TBS or PBS buffer if the kit is to be used for MS.
• Optimized buffers and Epoxy-coated Dynabeads® included in the kit
• Easy-to-use protocol with minimal hands-on time
• Covalent antibody coupling to the Dynabeads® avoids co-elution of the antibody with the target protein
Efficient, easy coupling
Antibodies are covalently coupled to Dynabeads® M-270 Epoxy beads, minimizing the risk of bound antibody contaminating your final eluate. Dynabeads® M-270 Epoxy beads exhibit ultra-low background binding and don't require blocking before use. Other proteins such as lectins and enzymes may also be coupled using the same beads, buffers, and protocol (depending on the stability and functionality of the protein/ligand you are using).
Magnetic bead-based separation offers easy handling
Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag™ magnet and the magnetic separation properties of the Dynabeads® . The supplied buffers are optimized to give consistent and reliable results and the kit includes 5 different binding, washing, and elution buffers, in addition to Dynabeads® M-270 Epoxy. The only thing you need to supply is the antibody of choice.
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