Genome Qualified:Promega's Genome-Qualified restriction enzymes are assayed to ensure optimal performance in genomic analysis applications.
Notes
Sensitive todammethylation.
For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994)Nucl. Acids Res.22, 3640–59.
Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.