收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

Cell Meter™ Nuclear Apoptosis Assay Kit *Green Fluorescence*

 
包装: 1 kit
运保温度: Freeze (<-15 °C) Avoid light
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

其它组分:

200X Nuclear Green™DCS1【包装:1 vial (500 μL)】
Assay Buffer【包装:1 bottle (100 mL)】


描述:

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the apoptotic chromatin condensation. The compacted chromatin of apoptotic cells binds higher amounts of nuclear dye compared to the healthy cells. Our Cell Meter™ Nuclear Apoptosis Assay Kit provides all the essential components with an optimized assay method for the detection of apoptosis in cells with condensed nuclei. This fluorometric assay is based on the detection of the DNA contents in cells using our proprietary non-fluorescent dye that becomes strongly fluorescent upon binding to DNA. In normal cells, Nuclear Green™ is not cell permeable, however, in apoptotic cells, Cells with compromised plasma membranes or with impaired/no cell metabolism are unable to prevent the dye from entering the cell. Once inside the cell, the dyes bind to intracellular DNA producing highly fluorescent complexes which identify the cells as non-viable cells. The staining with Nuclear Green™ DCS1 can be measured using a flow cytometer with Ex/Em = 490 nm/520 nm (FL1 channel) or fluorescence microscope (FITC filter set). The kit can be used with our other apoptosis reagents, such as Our Cell Meter™ NIR Mitochondria Membrane Potential Detection Kit (Cat# 22802), for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening of apoptosis activators and inhibitors.


相关图片

说明书

参考文献

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错