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3-(N-吗啡啉)丙磺酸(MOPS)MOPS

 
包装: 25 g
运保温度: Room Temperature
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描述:

3-[N-Morpholino]propanesulfonic acid
Molecular Biology Reagent
Purity: 99+%
Free Acid
Useful buffer range: 6.5-7.9

Description: MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers.1 It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7.4.

A buffer using MOPS free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa ± 1), using about a half-equivalent of NaOH. A buffer using MOPS sodium salt can be made by mixing equimolar MOPS free acid and MOPS sodium salt solutions to attain a buffer of the desired pH. Titration of a solution of MOPS sodium salt with HCl results in a solution that will contain NaCl, so the ionic strength will be higher than appropriate for some applications.

Concentrations higher than 20 mM should not be used with mammalian cell cultures.

 

3-(N-吗啡)丙烷磺酸;3-(N-吗啉基)丙磺酸
用途:生物缓冲液

适用于叶绿体薄层备样的电子传递和磷酸化研究;可以配制成多种琼脂培养基,在极限培养基中作为无毒缓冲液应用于棒状链霉菌(Streptomyces clavuligerus)的培养和头孢菌素(cephalosporin)的生产;可作为二维凝胶电泳中等电聚焦电泳(IEF)的电解质系统成分;还可应用于Northern杂交,作为RNA的分离和转膜时的缓冲液。

名称:3-(N-吗啡啉)丙磺酸 MOPS(有配好的缓冲液)

   英文:MOPS 3(N-MORPHOLINO)PROPANE-SULFONIC ACID

   CAS:1132-61-2

   介绍:MOPS是一种两性离子缓冲液,主要用用于配制作RNA 变性电泳缓冲液,缓冲范围是:6.5-7.9。在20mM的甲醛胶中作用尤为出色。多酚氧化酶(PPO)的活性测定会用到。


注意事项:

The pH of a 0.1 M solution of the sodium salt is approximately 10-12 and a 0.1 M solution of the free acid is approximately pH 2.5 to 4.0, depending on temperature. Solutions can be stored refrigerated for tup to approximately 6 months. Solutions should not be autoclaved1; sterilization of solutions should be done by filter sterilization through a 0.2 um filter.
 


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