蛋白酶K,来源于源于白色念球菌Proteinase K from Tritirachium Album

• 产品编号：0219350480      品牌：MP       原厂货号：0219350480
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 蛋白酶 > 蛋白酶K
• 应用分类：核酸分离纯化与分析 > DNA纯化 > 基因组 DNA 纯化
  　　　　　细胞分析 > 细胞分析研究平台 > 原位杂交 (ISH) > 荧光原位杂交 (FISH)

描述：

From Tritirachium album
Chromatographically purified
Suitable for both protein and nucleic acid isolation. Exhibits proteolytic activity on proteins, peptides, glycoproteins, amides and esters. Also active with nitroanilides of amino acids with protected amino groups, excluding arginine. Useful in the isolation of DNA and RNA, in the analysis of membrane structures and protein structure.
Proteolytic Activity: >30mAnson Units/mg
Unit Definition: One Anson unit is the amount of enzyme which liberates 1 μmol of Folin-positive amino acids per minute at pH 7.5 and 35°C, using hemoglobin as substrate.
Protein Content: >80%
RNase: < 5 x 10^{-4up> U/mg
DNase: < 5 x 10-4 U/mg... Read More}

蛋白酶K在原位杂交技术中通常用于杂交前的处理,它具有消化包围靶DNA蛋白质的作用,以增加探针与靶核酸结合的机会,提高杂交信号.但蛋白酶K的浓度过高、消化时间过长或孵育温度过高时,都会对细胞的结构有一定的破坏,导致组织切片的脱落,细胞核的消失,从而影响杂交结果.EDTA缓冲液可以替代蛋白酶K的作用,解决上述出现的问题,并能达到理想的染色效果.

制备脉冲电泳的染色体DNA，蛋白质印迹以及去除DNA和RNA制备中的核酸酶

原厂资料：