BD Pharmingen™ Red FP Vector - Mitochondrion is a mammalian expression vector that encodes a fusion of the red fluorescent protein (FP)
from Discosoma sp. with the mitochondrial targeting sequence from the subunit VIII of the human cytochrome c oxidase. The mitochondrial
targeting sequence is fused to the 5’-end of red FP. In order to increase the translation efficiency in mammalian cells, a Kozak consensus
translation initiation site has been introduced to the 5’-end of the red FP open reading frame, and its sequence has been optimized with human
codons. The red FP fusion is expressed under the control of the immediate early promoter of cytomegalovirus (P CMV ie), and its sequence is
followed by downstream simian virus 40 (SV40) polyadenylation signals. The vector contains a neomycin resistance gene controlled by the
SV40 promoter (P SV40 e) that allows selection of stably transfected eukaryotic cells using G418. An additional bacterial (Kan R) promoter
drives the expression of the same gene encoding for kanamycin resistance in E. coli.