Myb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5'-YAAC[GT]G-3'. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Product Information
Format
Purified
Control
Jurkat cell lysate.
Included Positive Antigen Control: Catalog # 12-303, Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.
Applications
Application
Anti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.
Key Applications
Immunohistochemistry
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.
FUNCTION: SwissProt: P10242 # Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
SIZE: 640 amino acids; 72341 Da
SUBUNIT: Binds MYBBP1A. Interacts with HIPK2 and NLK (By similarity).
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: P10242 Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C- terminal domain involved in transcriptional repression.
PTM: Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation (Probable). & Phosphorylated by NLK on multiple sites, which induces proteasomal degradation (By similarity).
SIMILARITY: Contains 3 HTH myb-type DNA-binding domains.
Product Usage Statements
Quality Assurance
Routinely evaluated on RIPA lysate from Jurkat cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Myb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5'-YAAC[GT]G-3'. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Product Information
Format
Purified
Control
Jurkat cell lysate.
Included Positive Antigen Control: Catalog # 12-303, Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.
Applications
Application
Anti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.
Key Applications
Immunohistochemistry
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.
FUNCTION: SwissProt: P10242 # Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
SIZE: 640 amino acids; 72341 Da
SUBUNIT: Binds MYBBP1A. Interacts with HIPK2 and NLK (By similarity).
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: P10242 Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C- terminal domain involved in transcriptional repression.
PTM: Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation (Probable). & Phosphorylated by NLK on multiple sites, which induces proteasomal degradation (By similarity).
SIMILARITY: Contains 3 HTH myb-type DNA-binding domains.
Product Usage Statements
Quality Assurance
Routinely evaluated on RIPA lysate from Jurkat cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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