Protein-tyrosine phosphatase of regenerating liver 2
Background Information
PRL-2 is one of three members (PRL-1, PRL-2, and PRL-3) that is part of a unique protein-tyrosine phosphatase ((PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP is known to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferres a transformed phenotype and can result in a tumor growth in nude mice, suggesting a role in tumorigenesis. Alternatively spliced transcript variants that encode two distinct isoforms have been described
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-PRL-2 Antibody, clone 42 detects level of PRL-2 & has been published & validated for use in WB, IH & IP.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis:
A representative lot was used by an independent laboratory in IH.
Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in IP.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse PRL-2.
Epitope
Unknown
Clone
42
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes PRL-2.
Isotype
IgG2bκ
Species Reactivity
Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
FUNCTION:Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyltransferase type II activity by blocking the association between RABGGTA and RABGGTB By similarity. CATALYTIC ACTIVITY:Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. ENZYME REGULATION:Inhibited by sodium orthovanadate and pentamidine By similarity. SUBUNIT STRUCTURE:In contrast to PTP4A1 and PTP4A3, does not interact with tubulin. Interacts with RABGGTB By similarity. SUBCELLULAR LOCATION: Cell membrane. Early endosome. Cytoplasm TISSUE SPECIFICTY:Expressed in skeletal muscle, and at lower levels in liver, lung, heart, kidney, brain, testis and spleen. Ref.1 PTM:Farnesylated. Farnesylation is required for membrane targeting and for interaction with RABGGTB By similarity. SEQUENCE SIMILARITIES:Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected PRL-2 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, M
WB, IP, IHC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1583
Description
Anti-PRL-2 Antibody, clone 42
Alternate Names
Protein-tyrosine phosphatase 4a2
protein tyrosine phosphatase type IVA, member 2
protein tyrosine phosphatase IVA2
protein tyrosine phosphatase IVA
phosphatase of regenerating liver 2
Protein-tyrosine phosphatase of regenerating liver 2
Background Information
PRL-2 is one of three members (PRL-1, PRL-2, and PRL-3) that is part of a unique protein-tyrosine phosphatase ((PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP is known to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferres a transformed phenotype and can result in a tumor growth in nude mice, suggesting a role in tumorigenesis. Alternatively spliced transcript variants that encode two distinct isoforms have been described
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-PRL-2 Antibody, clone 42 detects level of PRL-2 & has been published & validated for use in WB, IH & IP.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis:
A representative lot was used by an independent laboratory in IH.
Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in IP.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse PRL-2.
Epitope
Unknown
Clone
42
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes PRL-2.
Isotype
IgG2bκ
Species Reactivity
Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
FUNCTION:Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyltransferase type II activity by blocking the association between RABGGTA and RABGGTB By similarity. CATALYTIC ACTIVITY:Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. ENZYME REGULATION:Inhibited by sodium orthovanadate and pentamidine By similarity. SUBUNIT STRUCTURE:In contrast to PTP4A1 and PTP4A3, does not interact with tubulin. Interacts with RABGGTB By similarity. SUBCELLULAR LOCATION: Cell membrane. Early endosome. Cytoplasm TISSUE SPECIFICTY:Expressed in skeletal muscle, and at lower levels in liver, lung, heart, kidney, brain, testis and spleen. Ref.1 PTM:Farnesylated. Farnesylation is required for membrane targeting and for interaction with RABGGTB By similarity. SEQUENCE SIMILARITIES:Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected PRL-2 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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