Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.
Product Information
Format
Purified
Control
SW-480 cell lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.
Epitope
N-terminus
Clone
A5
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the N-terminus of Axin-1.
Isotype
IgG2aκ
Species Reactivity
Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
FUNCTION: Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of beta-catenin and APC by GSK3B. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 By similarity. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation.
SUBUNIT STRUCTURE: Homodimer. Interacts with GSK3B and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ternary complex. Also binds to plakoglobin (gamma-catenin), APC, DVL and PP2A. Interacts with SMAD6, SMAD7 and RNF111 By similarity. Interacts with TP53 and HIPK2. Probably part of a complex consisting of TP53, HIPK2 and AXIN1. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4, MDFI and MDFIC. Interacts with ANKRD6. Interacts with AIDA.
SUBCELLULAR LOCATION: Cytoplasm By similarity.
TISSUE SPECIFICITY: Expressed in embryonic stem cells.
DEVELOPMENTAL STAGE: Widely expressed at E10.5 to E16.5 day.
PTM: Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
SEQUENCE SIMILARITIES: Contains 1 DIX domain.
Contains 1 RGS domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, M
WB, ICC, IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1579
Description
Anti-Axin-1 Antibody, clone A5
Alternate Names
Axis inhibition protein 1
fused, mouse, homolog of
axis inhibitor 1
axin 1
Background Information
Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.
Product Information
Format
Purified
Control
SW-480 cell lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.
Key Applications
Western Blotting
Immunocytochemistry
Immunoprecipitation
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.
Epitope
N-terminus
Clone
A5
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the N-terminus of Axin-1.
Isotype
IgG2aκ
Species Reactivity
Human Mouse
Species Reactivity Note
Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
FUNCTION: Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of beta-catenin and APC by GSK3B. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 By similarity. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation.
SUBUNIT STRUCTURE: Homodimer. Interacts with GSK3B and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ternary complex. Also binds to plakoglobin (gamma-catenin), APC, DVL and PP2A. Interacts with SMAD6, SMAD7 and RNF111 By similarity. Interacts with TP53 and HIPK2. Probably part of a complex consisting of TP53, HIPK2 and AXIN1. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4, MDFI and MDFIC. Interacts with ANKRD6. Interacts with AIDA.
SUBCELLULAR LOCATION: Cytoplasm By similarity.
TISSUE SPECIFICITY: Expressed in embryonic stem cells.
DEVELOPMENTAL STAGE: Widely expressed at E10.5 to E16.5 day.
PTM: Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
SEQUENCE SIMILARITIES: Contains 1 DIX domain.
Contains 1 RGS domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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