Septin-6 is a filament-forming GTPase that participates in cytokenisis and is necessary for the normal organization of the actin cytoskeleton. It is widely expressed in tissues and is found in the cytoplasm and in the spindle during metaphase. Four isoforms of Septin-6 have been described.
As a protein family Septins were first characterized as regulators of cytokinesis. These proteins were later shown to have a broader role in microtubule-dependent processes, such as karyokinesis, exocytosis, and maintenance of cell shape. Several septin family members are known to colocalize or interact with the microtubule cytoskeleton and are believed to play a role in regulating microtubule dynamics through interactions with microtubule-associated proteins (MAPs).
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-Septin-6 Antibody, clone 9E7 is a Mouse Monoclonal Antibody for the detection of Septin-6 & has been validated in WB & IP.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Kremer, B., et al. (2005). MBC. 16(10):4648-4659.)
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Septin-6.
Epitope
Unknown
Clone
9E7
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes Septin-6.
Isotype
IgG3κ
Species Reactivity
Human Canine
Species Reactivity Note
Demonstrated to react with human. Predicted to react with canine based on 100% sequence homology.
This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. [provided by RefSeq].
FUNCTION: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication.
SUBUNIT STRUCTURE: Septins polymerize into hetero-oligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Filaments are assembled from asymmetrical heterotrimers, composed of SEPT2, SEPT6 and SEPT7 that associate head-to-head to form a hexameric unit. Within the trimer, directly interacts with SEPT2 and SEPT7. Also interacts with SEPT9 and SEPT12. Interaction with SEPT12 alters filament structure. Interacts with SOCS7. Interacts with HCV NS5B and with HNRNPA1.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton › spindle. Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle.
TISSUE SPECIFICITY: Widely expressed.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
MISCELLANEOUS: Coordinated expression with SEPT2 and SEPT7.
SEQUENCE SIMLARITIES: Belongs to the septin family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/ml of this antibody detected Septin-6 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µL
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, Ca
WB, IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1566
Description
Anti-Septin-6 Antibody, clone 9E7
Alternate Names
septin 6
septin 2
Background Information
Septin-6 is a filament-forming GTPase that participates in cytokenisis and is necessary for the normal organization of the actin cytoskeleton. It is widely expressed in tissues and is found in the cytoplasm and in the spindle during metaphase. Four isoforms of Septin-6 have been described.
As a protein family Septins were first characterized as regulators of cytokinesis. These proteins were later shown to have a broader role in microtubule-dependent processes, such as karyokinesis, exocytosis, and maintenance of cell shape. Several septin family members are known to colocalize or interact with the microtubule cytoskeleton and are believed to play a role in regulating microtubule dynamics through interactions with microtubule-associated proteins (MAPs).
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-Septin-6 Antibody, clone 9E7 is a Mouse Monoclonal Antibody for the detection of Septin-6 & has been validated in WB & IP.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Kremer, B., et al. (2005). MBC. 16(10):4648-4659.)
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Septin-6.
Epitope
Unknown
Clone
9E7
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes Septin-6.
Isotype
IgG3κ
Species Reactivity
Human Canine
Species Reactivity Note
Demonstrated to react with human. Predicted to react with canine based on 100% sequence homology.
This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. [provided by RefSeq].
FUNCTION: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication.
SUBUNIT STRUCTURE: Septins polymerize into hetero-oligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Filaments are assembled from asymmetrical heterotrimers, composed of SEPT2, SEPT6 and SEPT7 that associate head-to-head to form a hexameric unit. Within the trimer, directly interacts with SEPT2 and SEPT7. Also interacts with SEPT9 and SEPT12. Interaction with SEPT12 alters filament structure. Interacts with SOCS7. Interacts with HCV NS5B and with HNRNPA1.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton › spindle. Note: In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle.
TISSUE SPECIFICITY: Widely expressed.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
MISCELLANEOUS: Coordinated expression with SEPT2 and SEPT7.
SEQUENCE SIMLARITIES: Belongs to the septin family.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/ml of this antibody detected Septin-6 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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