PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Product Information
Format
Purified
Control
PC-12 cell lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application
This Anti-PI3 Kinase b Antibody, p110b, clone 8C3.2 is validated for use in WB, IC for the detection of PI3 Kinase b.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution positively stained NIH3T3 cell lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human PI3 Kinase b.
Epitope
N-terminus
Clone
8C3.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes PI3 Kinase b at the N-terminus.
Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM].
SUBUNIT STRUCTURE: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.
TISSUE SPECIFICITY: Expressed ubiquitously.
SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in PC-12 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PI3 Kinase b on 10 µg of PC-12 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Product Information
Format
Purified
Control
PC-12 cell lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Applications
Application
This Anti-PI3 Kinase b Antibody, p110b, clone 8C3.2 is validated for use in WB, IC for the detection of PI3 Kinase b.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:500 dilution positively stained NIH3T3 cell lysate.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the N-terminus of human PI3 Kinase b.
Epitope
N-terminus
Clone
8C3.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes PI3 Kinase b at the N-terminus.
Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM].
SUBUNIT STRUCTURE: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit.
TISSUE SPECIFICITY: Expressed ubiquitously.
SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in PC-12 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PI3 Kinase b on 10 µg of PC-12 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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