Gemin4, also known as Component of Gems 4, is a component of the SMN complex. It is found in the cytoplasm, nucleoli, and to nuclear bodies called Gemini bodies (gems). As part of a complex this protein functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. Gemin4 directly interacts with the DEAD box protein, Gemin3, as well as other snRNP proteins, including SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE.
The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence found in small nuclear RNAs. Reduced levels of SMN cause the motor neuron degenerative disease, spinal muscular atrophy.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-Gemin4 Antibody, clone 17D10 (mouse monoclonal antibody) validated in WB, IP to detect Gemin4 also known as HCC-associated protein 1, component of gems 4, gem (nuclear organelle) associated protein 4.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation:
Reported by external laboratory using representative lot of this antibody.
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to human Gemin4.
Epitope
Unknown
Clone
17D10
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
FUNCTION: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
SUBUNIT STRUCTURE: Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN3 and with several snRNP SM core proteins, including SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE. Interacts with PPP4R2.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus › nucleolus. Nucleus › gem. Note: Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs and in the nucleolus.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Gemin4 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
WB, IP
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1534
Description
Anti-Gemin4 Antibody, clone 17D10
Alternate Names
HCC-associated protein 1
gemin 4
gem (nuclear organelle) associated protein 4
component of gems 4
Background Information
Gemin4, also known as Component of Gems 4, is a component of the SMN complex. It is found in the cytoplasm, nucleoli, and to nuclear bodies called Gemini bodies (gems). As part of a complex this protein functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. Gemin4 directly interacts with the DEAD box protein, Gemin3, as well as other snRNP proteins, including SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE.
The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence found in small nuclear RNAs. Reduced levels of SMN cause the motor neuron degenerative disease, spinal muscular atrophy.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-Gemin4 Antibody, clone 17D10 (mouse monoclonal antibody) validated in WB, IP to detect Gemin4 also known as HCC-associated protein 1, component of gems 4, gem (nuclear organelle) associated protein 4.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation:
Reported by external laboratory using representative lot of this antibody.
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to human Gemin4.
Epitope
Unknown
Clone
17D10
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
FUNCTION: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.
SUBUNIT STRUCTURE: Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN3 and with several snRNP SM core proteins, including SNRPB, SNRPD1, SNRPD2, SNRPD3 and SNRPE. Interacts with PPP4R2.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus › nucleolus. Nucleus › gem. Note: Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs and in the nucleolus.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Gemin4 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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