The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. While all hnRNPs are present in the nucleus, data suggests they shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by hnRNP A2/B1 belongs to the A/B subfamily of ubiquitously express hnRNPs. This family member has two repeats of quasi-RRM domains that bind to RNAs. Two alternatively spliced transcript variants which encode different isoforms have been described. In autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, and mixed connective tissue disease hnRNPA2B1 can serve as an autoantigen. Although this protein is predominantly nucleoplasmic, in some tissues isoform A2 is found in the cytoplasm.
Product Information
Format
Purified
Control
HeLa nuclear extract
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-hnRNP A2/B1 Antibody, clone DP383 (Mouse Monoclonal Antibody) validated in WB, IHC, IP to detect hnRNP A2/B1 also known as heterogeneous nuclear ribonucleoprotein A2/B1.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Immunohistochemistry:
Cited in Mizukami, K., et al. (2000) Neuroreport. 11(14):3099-102 using a representative lot of this antibody.
Immunoprecipitation:
Cited in Kamma, H., et al. (1999) Exp. Cell Res. 246(2):399-411using a representative lot of this antibody.
Biological Information
Immunogen
Recombinant protein corresponding to human hnRNP A2/B1.
Epitope
Unknown
Clone
DP383
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq].
FUNCTION: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1.
SUBCELLULAR LOCATION: Nucleus › nucleoplasm. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus. Ref.15
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/ml of this antibody detected hnRNP A2/B1 on 10 µg of HeLa nuclear extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
WB, IP, IHC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1522
Description
Anti-hnRNP A2/B1 Antibody, clone DP383
Alternate Names
heterogeneous nuclear ribonucleoprotein A2/B1
nuclear ribonucleoprotein particle A2 protein
hnRNP A2 / hnRNP B1
heterogeneous nuclear ribonucleoprotein B1
Background Information
The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. While all hnRNPs are present in the nucleus, data suggests they shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by hnRNP A2/B1 belongs to the A/B subfamily of ubiquitously express hnRNPs. This family member has two repeats of quasi-RRM domains that bind to RNAs. Two alternatively spliced transcript variants which encode different isoforms have been described. In autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, and mixed connective tissue disease hnRNPA2B1 can serve as an autoantigen. Although this protein is predominantly nucleoplasmic, in some tissues isoform A2 is found in the cytoplasm.
Product Information
Format
Purified
Control
HeLa nuclear extract
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Use Anti-hnRNP A2/B1 Antibody, clone DP383 (Mouse Monoclonal Antibody) validated in WB, IHC, IP to detect hnRNP A2/B1 also known as heterogeneous nuclear ribonucleoprotein A2/B1.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Immunohistochemistry:
Cited in Mizukami, K., et al. (2000) Neuroreport. 11(14):3099-102 using a representative lot of this antibody.
Immunoprecipitation:
Cited in Kamma, H., et al. (1999) Exp. Cell Res. 246(2):399-411using a representative lot of this antibody.
Biological Information
Immunogen
Recombinant protein corresponding to human hnRNP A2/B1.
Epitope
Unknown
Clone
DP383
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq].
FUNCTION: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.
SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1.
SUBCELLULAR LOCATION: Nucleus › nucleoplasm. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus. Ref.15
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/ml of this antibody detected hnRNP A2/B1 on 10 µg of HeLa nuclear extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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