Transportin 1 (Trn1) is a transport receptor that transports substrates from the cytoplasm to the nucleus through nuclear pore complexes by recognizing nuclear localization signals (NLSs). Transportin 1 is a mediator of hnRNP A1 transport into the nucleus.
Trn1 has two sites for binding NLSs, one with high affinity and second with low affinity. Interactions at the low affinity site controls overall binding affinity. Trn1 recognizes NLSs at the high affinity site followed by a conformational change at the low affinity site that interacts with NLSs. Alternate splicing of the Trn1 gene results in two transcript variants encoding different proteins.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-Transportin 1 Antibody, clone D45 is a Mouse Monoclonal Antibody for detection of Transportin 1 also known as karyopherin (importin) beta 2 & has been validated in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to human Transportin-1.
Epitope
Unknown
Clone
D45
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Transportin-1.
Isotype
IgG1κ
Species Reactivity
Human Rabbit Monkey
Species Reactivity Note
Demonstrated to react with human. Predicted to react with monkey and rabbit based on 100% sequence homology.
This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq].
FUNCTION: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity. Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
SUBUNIT STRUCTURE: Binds HNRPA1, HNRPA2, HNRPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with H2A, H2B, H3 and H4 histones. Binds to HIV-1 Rev.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
SEQUENCE SIMILARITIES: Belongs to the importin beta family.
Contains 8 HEAT repeats.
Contains 1 importin N-terminal domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Transportin-1 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H, Rb, Mk
WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1515
Description
Anti-Transportin 1 Antibody, clone D45
Alternate Names
Importin beta-2
transportin 1
karyopherin (importin) beta 2
importin beta 2
importin 2
M9 region interaction protein
Karyopherin beta-2
Background Information
Transportin 1 (Trn1) is a transport receptor that transports substrates from the cytoplasm to the nucleus through nuclear pore complexes by recognizing nuclear localization signals (NLSs). Transportin 1 is a mediator of hnRNP A1 transport into the nucleus.
Trn1 has two sites for binding NLSs, one with high affinity and second with low affinity. Interactions at the low affinity site controls overall binding affinity. Trn1 recognizes NLSs at the high affinity site followed by a conformational change at the low affinity site that interacts with NLSs. Alternate splicing of the Trn1 gene results in two transcript variants encoding different proteins.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-Transportin 1 Antibody, clone D45 is a Mouse Monoclonal Antibody for detection of Transportin 1 also known as karyopherin (importin) beta 2 & has been validated in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Histidine-tagged recombinant protein corresponding to human Transportin-1.
Epitope
Unknown
Clone
D45
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes Transportin-1.
Isotype
IgG1κ
Species Reactivity
Human Rabbit Monkey
Species Reactivity Note
Demonstrated to react with human. Predicted to react with monkey and rabbit based on 100% sequence homology.
This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq].
FUNCTION: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity. Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
SUBUNIT STRUCTURE: Binds HNRPA1, HNRPA2, HNRPDL, RPL23A, RPS7, RPL5, RAN and SRP19. Interacts with H2A, H2B, H3 and H4 histones. Binds to HIV-1 Rev.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
SEQUENCE SIMILARITIES: Belongs to the importin beta family.
Contains 8 HEAT repeats.
Contains 1 importin N-terminal domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Transportin-1 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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