MAD2A is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. MAD2A then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately cell death or disease.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-MAD2A Antibody, clone 17D10 (Mouse Monoclonal Antibody) validated in WB, IP to detect MAD2A also known as MAD2 mitotic arrest deficient-like protein 1 (yeast).
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated MAD2A from 100 μg of HEK293 cell lysate.
Biological Information
Immunogen
Recombinant protein corresponding to full length human MAD2A.
Clone
17D10
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION:Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. SUBUNIT STRUCTURE:Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. SUBCELLULAR LOCATION:Nucleus. Kinetochore. Cytoplasm. Note: Recruited by MAD1L1 to unattached kinetochores Probable. Recruited to the nuclear pore complex by TPR during interphase. DOMAIN:The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20. PTM:Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. SEQUENCE SIMILARITIES:Belongs to the MAD2 family.
Contains 1 HORMA domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MAD2A in 15 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
MAD2A is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. MAD2A then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately cell death or disease.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Use Anti-MAD2A Antibody, clone 17D10 (Mouse Monoclonal Antibody) validated in WB, IP to detect MAD2A also known as MAD2 mitotic arrest deficient-like protein 1 (yeast).
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated MAD2A from 100 μg of HEK293 cell lysate.
Biological Information
Immunogen
Recombinant protein corresponding to full length human MAD2A.
Clone
17D10
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION:Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. SUBUNIT STRUCTURE:Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. SUBCELLULAR LOCATION:Nucleus. Kinetochore. Cytoplasm. Note: Recruited by MAD1L1 to unattached kinetochores Probable. Recruited to the nuclear pore complex by TPR during interphase. DOMAIN:The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20. PTM:Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. SEQUENCE SIMILARITIES:Belongs to the MAD2 family.
Contains 1 HORMA domain.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MAD2A in 15 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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