PHD3 (Prolyl hydroxylase domain-containing protein 3), also known as Hypoxia-inducible factor prolyl hydroxylase 3 (HIF-PH3 or HPH-1), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. PHD3, is expressed in response to hypoxia and causes apoptosis in oxygenated conditions in neural cells. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Product Information
Format
Purified
Control
Human placenta tissue lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-PHD3 Antibody, clone 188e is a mouse monoclonal antibody for detection of PHD3 also known as EGL nine (C.elegans) homolog 3, HIF-prolyl hydroxylase 3, Hypoxia-inducible factor prolyl hydroxylase 3, & has been validated in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Recombinant protein corresponding to human PHD3.
Epitope
Unknown
Clone
188e
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism By similarity.
ENZYME REGULATION: Activated in cardiovascular cells and HelA cells following exposure to hypoxia.
SUBUNIT STRUCTURE: Interacts with MORG1 By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PHD3 on 10 µg of human placenta tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
原厂资料：
Key Spec Table
Species Reactivity
Key Applications
Host
Format
Antibody Type
H
WB
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-1328
Description
Anti-PHD3 Antibody, clone 188e
Alternate Names
EGL nine (C.elegans) homolog 3
egl nine-like protein 3 isoform
egl nine homolog 3 (C. elegans)
egl nine homolog 3
Prolyl hydroxylase domain-containing protein 3
Hypoxia-inducible factor prolyl hydroxylase 3
HIF-prolyl hydroxylase 3
HIF prolyl hydroxylase 3
Background Information
PHD3 (Prolyl hydroxylase domain-containing protein 3), also known as Hypoxia-inducible factor prolyl hydroxylase 3 (HIF-PH3 or HPH-1), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. PHD3, is expressed in response to hypoxia and causes apoptosis in oxygenated conditions in neural cells. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Product Information
Format
Purified
Control
Human placenta tissue lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application
Anti-PHD3 Antibody, clone 188e is a mouse monoclonal antibody for detection of PHD3 also known as EGL nine (C.elegans) homolog 3, HIF-prolyl hydroxylase 3, Hypoxia-inducible factor prolyl hydroxylase 3, & has been validated in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Recombinant protein corresponding to human PHD3.
Epitope
Unknown
Clone
188e
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism By similarity.
ENZYME REGULATION: Activated in cardiovascular cells and HelA cells following exposure to hypoxia.
SUBUNIT STRUCTURE: Interacts with MORG1 By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Widely expressed at low levels. Expressed at higher levels in heart (cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle) and placenta.
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PHD3 on 10 µg of human placenta tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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